Quantitation of Microorganisms in Sputum
نویسندگان
چکیده
منابع مشابه
Quantitation of sputum protein by use of the biuret reaction.
A simple, accurate method is described for quantitatively determining the protein content of sputum by use of the biuret reaction. Fitteen mechanically homogenized specimens were examined with the biuret reagent described by Rosenthal and Cundiff, and the results compared with those obtained by the microKjeldahl technique. Over a range of 542 to 1917 mg of protein per 100 ml, the techniques cor...
متن کاملThe detection and quantitation of free desmosine and isodesmosine in human urine and their peptide-bound forms in sputum.
Desmosine (D) and isodesmosine (I), the intramolecular crosslinking amino acids that occur in chains of elastin, have now been found in free form in human urine. Until now, these amino acids (M(r) = 526) were found to occur in urine only as higher molecular weight (M (r) = 1,000-1,500) peptides. Thus, the previously used analytical methods required, as the first step, acid hydrolysis of the uri...
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Background and Objectives: Sputum smear staining for acid-fast bacilli is initial approach to the diagnosis of pulmonary tuberculosis (PTB) but more than 50% of cases are sputum smear-negative. This study was aimed to investigate the diagnostic value of fiberoptic bronchoscopy (FOB) guided bronchoalveolar lavage (BAL) in patients suspected to have tuberculosis. Methods: This prospective cross-...
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The aim of this study is to develop a standardized LC-MS/MS method for accurate measurement of desmosine (DES) and isodesmosine (IDS) in all body fluids as biomarkers for in vivo degradation of matrix tissue elastin in man and animals. A reproducible three-step analytical procedure: (1) sample hydrolysis in 6N HCl, (2) SPE by a CF1 cartridge with addition of acetylated pyridinoline as internal ...
متن کاملQuantitation of pneumococcal C polysaccharide in sputum samples from patients with presumptive pneumococcal pneumonia by enzyme immunoassay.
Although the Gram stain and culture of expectorated sputum are considered standard methods for the diagnosis of presumptive pneumococcal pneumonia, these methods remain relatively insensitive and nonspecific. We developed an enzyme immunoassay (EIA) for the quantitation of pneumococcal C polysaccharide (PnC) in the sputum of patients with presumptive pneumococcal pneumonia. Of 34 patient sputum...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1969
ISSN: 0003-6919
DOI: 10.1128/aem.18.2.214-220.1969